human oc cell lines skov3  (ATCC)

Journal: Cell Death & Disease

Article Title: UHRF1-mediated HIF-1α stabilization promotes ovarian cancer through metabolic reprogramming and angiogenesis

doi: 10.1038/s41419-025-08033-w

Figure Lengend Snippet: A – D CCK8 assay to assess the effect of UHRF1-knockdown and UHRF1-overexpressed OC cell lines on the proliferation. E EDU cell proliferation assay showing the effect of UHRF1-knockdown OC cell lines. F Colony formation assay showing the effect of UHRF1-knockdown OC cell lines on colony-forming ability. G , H Transwell migration and invasion assays showing the effect of UHRF1-knockdown OC cell lines on migration and invasion abilities. I Wound healing assay showing UHRF1-knockdown SKOV3 cell lines migration. J Tumorigenicity assay in nude mice showing the effect of UHRF1-knockdown SKOV3 cell lines on tumor formation. K Ki67 staining of tumor tissues to assess the effect of UHRF1 knockdown on cell proliferation. ** p < 0.01; *** p < 0.001; **** p < 0.0001.

Article Snippet: The human OC cell line SKOV3 (CL-0215) was purchased from Wuhan Procell Life Science & Technology Co., Ltd., and A2780 (STCC10706P-1) was purchased from Wuhan Servicebio Technology Co., Ltd.

Techniques: CCK-8 Assay, Knockdown, Proliferation Assay, Colony Assay, Migration, Wound Healing Assay, Tumorigenicity Assay, Staining

Journal: Cell Death & Disease

Article Title: UHRF1-mediated HIF-1α stabilization promotes ovarian cancer through metabolic reprogramming and angiogenesis

doi: 10.1038/s41419-025-08033-w

Figure Lengend Snippet: A Volcano plot showing differential expression analysis between sh-NC SKOV3 cells and sh-UHRF1#2 SKOV3 cells, highlighting key potential downstream molecules. B Heatmap displaying the expression of HIF-1 signaling pathway genes between the knockdown and control groups. C , D KEGG pathway enrichment analysis of differentially expressed genes (DEGs), bubble chart shows the enriched pathways; circle plot displays the specific genes within the pathways. E GO-MF enrichment analysis of DEGs, the specific pathway names can be found in (Supplementary Table ). F GSEA enrichment analysis of DEGs. G Correlation analysis of UHRF1 and HIF-1 signaling pathway genes using GEPIA2.

Article Snippet: The human OC cell line SKOV3 (CL-0215) was purchased from Wuhan Procell Life Science & Technology Co., Ltd., and A2780 (STCC10706P-1) was purchased from Wuhan Servicebio Technology Co., Ltd.

Techniques: Quantitative Proteomics, Expressing, Knockdown, Control

Journal: Cell Death & Disease

Article Title: UHRF1-mediated HIF-1α stabilization promotes ovarian cancer through metabolic reprogramming and angiogenesis

doi: 10.1038/s41419-025-08033-w

Figure Lengend Snippet: A Tissue immunofluorescence (IF) confirmed the colocalization of UHRF1 and HIF-1α in tissues. B Silver staining of mass spectrometry samples. C Co-immunoprecipitation (co-IP) confirmed the interaction between UHRF1 and HIF-1α in SKOV3 cells. D Forward and reverse co-IP validated the interaction between UHRF1 and HIF-1α in 293T cells. E IB analysis of interaction domains between HIF-1α and UHRF1 using truncated plasmids co-transfected in 293T cells. F Truncated sequence map of UHRF1. G Truncated sequence map of HIF-1α. H WB analysis of interaction domains between UHRF1 and HIF-1α using truncated plasmids co-transfected in 293T cells. I WB analysis of UHRF1’s effect on the nuclear translocation of HIF-1α. J IF analysis of UHRF1’s effect on HIF-1α nuclear translocation.

Article Snippet: The human OC cell line SKOV3 (CL-0215) was purchased from Wuhan Procell Life Science & Technology Co., Ltd., and A2780 (STCC10706P-1) was purchased from Wuhan Servicebio Technology Co., Ltd.

Techniques: Immunofluorescence, Silver Staining, Mass Spectrometry, Immunoprecipitation, Co-Immunoprecipitation Assay, Transfection, Sequencing, Translocation Assay

Journal: Cell Death & Disease

Article Title: UHRF1-mediated HIF-1α stabilization promotes ovarian cancer through metabolic reprogramming and angiogenesis

doi: 10.1038/s41419-025-08033-w

Figure Lengend Snippet: A UMAP plot with clusters demarcated by colors demonstrating 12 distinct clusters based on gene expression differences for epithelial cells passing quality control. B The UMAP plot demarcated by colors showing the two groups of OC tumors (malignant) and nonmalignant ovarian tissues. C Expression of HIF signaling pathway genes in tumor and normal tissues. D Expression of HIF signaling pathway genes in each epithelial cell cluster. E UMAP plot color coded for the expression (blue to purple) of marker genes for HIF signaling pathway genes. F The proportion of epithelial cell types relative to the total epithelial cell count in each clinical patient. G KEGG pathway enrichment analysis of epithelial-cluster6 genes. H , I UHRF1-knockout and UHRF1-overexpressed SKOV3 cell lines were treated with CoCl₂ for 24 h, and WB analysis was performed to assess the expression levels of glycolysis-related proteins. J – M UHRF1-knockdown and UHRF1-overexpressed OC cell lines were cultured under normoxic and hypoxic conditions for 48 h, and glucose consumption and lactate production were measured. Data are presented as mean ± SD from three independent experiments. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.

Article Snippet: The human OC cell line SKOV3 (CL-0215) was purchased from Wuhan Procell Life Science & Technology Co., Ltd., and A2780 (STCC10706P-1) was purchased from Wuhan Servicebio Technology Co., Ltd.

Techniques: Gene Expression, Control, Expressing, Marker, Cell Counting, Knock-Out, Knockdown, Cell Culture

Journal: Cell Death & Disease

Article Title: UHRF1-mediated HIF-1α stabilization promotes ovarian cancer through metabolic reprogramming and angiogenesis

doi: 10.1038/s41419-025-08033-w

Figure Lengend Snippet: A , B Periodic acid-Schiff (PAS) (pink) and CD31 (brown) double staining of tumor tissues from xenograft mice. PAS+/CD31+ tubular structures represent conventional blood vessels lined with CD31-positive endothelial cells. Adjacent PAS+/CD31− tubular structures suggest vasculogenic mimicry (VM). C , D WB analysis of VEGF expression levels in cells treated with CoCl₂ for 24 h. E Representative images of the tube formation assay in SKOV3 stable cell lines. F Representative images of the tube formation assay in HUVEC cells after 72 h of culture with conditioned medium from the stable SKOV3 cell line. G Images of the Transwell migration assay and tube formation assay in HUVEC cells after 72 hours of culture with conditioned medium from the stable SKOV3 cell line. H , I Images of the Transwell migration assay and tube formation assay in HUVEC cells after 72 h of culture with conditioned medium from the stable SKOV3 cell line, followed by treatment with the HIF-1α inhibitor PX-478. J , K IC50 analysis of Bevacizumab in UHRF1-knockdown A2780 cell lines. ** p < 0.01; *** p < 0.001; **** p < 0.0001.

Article Snippet: The human OC cell line SKOV3 (CL-0215) was purchased from Wuhan Procell Life Science & Technology Co., Ltd., and A2780 (STCC10706P-1) was purchased from Wuhan Servicebio Technology Co., Ltd.

Techniques: Double Staining, Expressing, Tube Formation Assay, Stable Transfection, Transwell Migration Assay, Knockdown